New! 900 MHz Data from 1.6mm HFXY PhoenixNMR Probe

May 29th, 2020 Posted by PhoenixNMR, Products, Technology No Comment yet

Below: aliphatic region of the 2D CC spectrum of 15N, 13C labeled alpha-synuclein protein monomer seeded from a brain tissue sample (250 ms CORD mixing), acquired at 900 MHz 1H field at the National Magnetic Resonance Facility at Madison (NMRFAM, @NMRFAM) , utilizing a PhoenixNMR HFXY probe fitted with a 1.6mm module and PhoenixNMR speed controller (PMAS).

The PhoenixNMR HFXY probe was operated in double resonance mode at MASS = 20 kHz; mixing time = 250 ms; total time to acquire spectrum = 20 hrs using 4 blocks of 1024 rows each; 8 acquisitions per row; pulse delay = 1.5 s; CORDxy4 recoupling plus SPINAL decoupling.

Expansion of neardiagonal region of NMRFAM data acquired with PhoenixNMR probe
Expansion of a near-diagonal region of the above 2D CC spectrum
Expansion of Near Diagonal Region of NMRFAM spectrum acquired with PhoenixNMR HFXY probe operating at 900 MHz
A second Expansion of a near-diagonal region of the 2D CC Spectrum. Note the excellent lineshape provided by the PhoenixNMR probe demonstrated by the ability to observe these near-diagonal peaks in both of these expansions.

Data courtesy Prof. Chad M. Rienstra, Dr. Marco Tonelli and Dr. Dwaipayan Mukhopadhyay. Data were acquired at the National Magnetic Resonance Facility at Madison ( NMRFAM, @NMRFAM). This 900 MHz SSNMR instrument and probe are available for use at NMRFAM by any scientist worldwide.

For more information about the PhoenixNMR HFXY Probe or PMAS Controller:

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